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Core technical expertise

Our expertise is centered on electrophysiology and complemented by other state of the art techniques to study MOAs in detail.

Patch-clamp

Whole-cell recordings allow a precise measurement of a single cell electrical activity by detecting sub-millisecond variations in membrane potential

  • Extremely precise data on single cell physiology.

  • It can be used in any brain or spinal cord structure and allows targeted recordings of specific cell types.

  • Allow targeted pre or post synaptic manipulations specifically.

  • Essential when studying the mechanisms of action and pharmacological profile of a compound.

Extracellular recordings

Field recordings are used to extracellularly record the activity of a population of neurons, or field potentials.

  • Several localized neuronal populations are recorded simultaneously in a given slice.

  • Faster than patch-clamp, allows for a quick screening of several compounds or doses at mid-throughput data production.

Calcium imaging

Calcium imaging can be combined to extra- or intra-cellular recordings or be employed alone. This approach measures the activity of individual cells (e.g. expressing a calcium-dependent fluorescent dye) in culture or in slices via the identification of calcium transients. Calcium is indeed a hallmark of neuronal activity as neurotransmitter release requires significant increase in intracellular calcium levels.

  • Can be used in slices or in cultures and combined to extracellular recordings or patch-clamp

  • Complementary approach to determine the effect of test compounds on neuronal activity and calcium channels in multiple locations simultaneously

Other techniques

Our capabilities include histology/immunochemistry and pharmacology as well as optogenetics combined with electrophysiology. Manipulations can be extracellular or intracellular (when using patch-clamp).

 

Depending on the context, we can perform recordings on specific transgenic animal models and employ acute or chronic compound administration. ​