Core technical expertise
Our expertise is centered on electrophysiology and complemented by other state of the art techniques to study MOAs in detail.
Whole-cell recordings allow a precise measurement of a single cell electrical activity by detecting sub-millisecond variations in membrane potential
Extremely precise data on single cell physiology.
It can be used in any brain or spinal cord structure and allows targeted recordings of specific cell types.
Allow targeted pre or post synaptic manipulations specifically.
Essential when studying the mechanisms of action and pharmacological profile of a compound.
Field recordings are used to extracellularly record the activity of a population of neurons, or field potentials.
Several localized neuronal populations are recorded simultaneously in a given slice.
Faster than patch-clamp, allows for a quick screening of several compounds or doses at mid-throughput data production.
Calcium imaging can be combined to extra- or intra-cellular recordings or be employed alone. This approach measures the activity of individual cells (e.g. expressing a calcium-dependent fluorescent dye) in culture or in slices via the identification of calcium transients. Calcium is indeed a hallmark of neuronal activity as neurotransmitter release requires significant increase in intracellular calcium levels.
Can be used in slices or in cultures and combined to extracellular recordings or patch-clamp
Complementary approach to determine the effect of test compounds on neuronal activity and calcium channels in multiple locations simultaneously
Our capabilities include histology/immunochemistry and pharmacology as well as optogenetics combined with electrophysiology. Manipulations can be extracellular or intracellular (when using patch-clamp).
Depending on the context, we can perform recordings on specific transgenic animal models and employ acute or chronic compound administration.