Human neuronal culture
Animal-to-human data translation is particularly challenging in the central nervous system area.
We therefore propose to use human neurons derived from human induced-pluripotent stem cells (iPSCs) to complement and confirm the electrophysiology data obtained from rodents. This combination reinforces confidence in the data and limits the risks before engaging in the next steps.
with data from human neurons
for specific pathologies
A powerful new way to optimize your studies
IPSC-derived neurons are a new and powerful tool to assess the safety and efficacy profiles of candidate compounds. The are usually reprogrammed human fibroblasts that can then be re-differentiated into different neuronal types.
Other than their human origin, a key advantage of these neurons resides in the fact that they may come from patients carrying a specific mutation of interest for a particular pathology.
IPSCs can be differentiated into the most common cell types (glutamatergic, GABAergic, dopaminergic, motor neurons etc.) and are selected based on the specificities of each project.
Although a more complex preparation is required, the patch-clamp recordings themselves do not take more time or effort than on tissues from rodents.
Because of their human origin they complement and reinforce results obtained from rodents, leading to more reliable and predictive results.